protective effects of interleukin-4 on tissue destruction and morphological changes of bovine nasal chondrocytes in vitro
نویسندگان
چکیده
background: previous studies have shown that some cytokines have protective effects on cartilage in joint diseases. in the current study, effects of il-4 against morphological changes and tissue degradation induced by il-1α on bovine nasal cartilage (bnc) explants were investigated. methods: fresh bnc samples were prepared from a slaughterhouse under sterile conditions. bnc explants culture was treated with both il-lα (10 ng/ml) and il-4 (50 ng/ml) at the same time for 28 days. the morphological characteristics of explants were assessed by using histology techniques and invert microscopy. matrix metalloproteinase-1 (mmp-1) production was assessed within different days by using western blotting. results: il-lα induced prominent cartilage morphology degradation. the pro and active form of mmp-1 band substantially increased at day 21 of culture. in the presence of both il-lα and il-4, chondrocytes preserved their ordinary normal phenotype with intact extracellular matrix. in addition, a significant reduction in pro-mmp-1and inhibition of active mmp-1 was seen. conclusion: in conclusion, il-4 could be regarded as a potential candidate in cartilage protecting against the degradation changes of il-lα. it seems that the preservation effect of il-4 is associated with significant reduction of mmp-1.
منابع مشابه
Protective Effects of Interleukin-4 on Tissue Destruction and Morphological Changes of Bovine Nasal Chondrocytes in vitro
Background: Previous studies have shown that some cytokines have protective effects on cartilage in joint diseases. In the current study, effects of IL-4 against morphological changes and tissue degradation induced by IL-1α on bovine nasal cartilage (BNC) explants were investigated. Methods: Fresh BNC samples were prepared from a slaughterhouse under sterile conditions. BNC explants culture was...
متن کاملProtective effects of interleukin-4 on tissue destruction and morphological changes of bovine nasal chondrocytes in vitro.
BACKGROUND Previous studies have shown that some cytokines have protective effects on cartilage in joint diseases. In the current study, effects of IL-4 against morphological changes and tissue degradation induced by IL-1α on bovine nasal cartilage (BNC) explants were investigated. METHODS Fresh BNC samples were prepared from a slaughterhouse under sterile conditions. BNC explants culture was...
متن کاملmorphological changes of bovine nasal chondrocytes induced by interleukin-1α
objective: a study of the histological events under interleukin-1α (il-lα) induction of bovine nasal cartilage (bnc) could result in useful data to better understand the mechanisms involved in tissue breakdown in joint diseases. the aim of this study was to investigate the effects of il-lα on chondrocyte phenotype and extracellular matrix (ecm) changes in bnc explants. materials and methods: in...
متن کاملMorphological Changes of Bovine Nasal Chondrocytes Induced by Interleukin-1α
OBJECTIVE A study of the histological events under interleukin-1α (IL-lα) induction of bovine nasal cartilage (BNC) could result in useful data to better understand the mechanisms involved in tissue breakdown in joint diseases. The aim of this study was to investigate the effects of IL-lα on chondrocyte phenotype and extracellular matrix (ECM) changes in BNC explants. MATERIALS AND METHODS In...
متن کاملCombination Effects of Prednisolone and Interleukin-4 Protect Bovine Nasal Cartilage Explants from Interleukin-1α Induced Degradation
Background: Current treatments for joint diseases are moderately successful, but unfortunately are associated with significant side effects. This study was undertaken to investigate the combination effects of IL-4 and prednisolone on tissue characteristics and production of matrix metalloproteinase-1(MMP-1) in IL-lα-treated bovine nasal cartilage (BNC) explants. Methods: BNC explants were cultu...
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عنوان ژورنال:
iranian biomedical journalجلد ۱۷، شماره ۴، صفحات ۱۸۷-۱۹۳
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